Journal: Cellular and Molecular Immunology

Article Title: Naturally occurring spike mutations influence the infectivity and immunogenicity of SARS-CoV-2

doi: 10.1038/s41423-022-00924-8

Figure Lengend Snippet: Substitution of S982A and L18F weakens the generation of binding antibodies. The area under the curve (AUC) of anti-spike ( A ) and anti-RBD ( B ) IgG induced by the 19 designed groups of DNA immunogens ( n = 3 for each group). The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8. Data were analyzed for statistical significance using the unpaired Student’s t test. Data are shown as the mean with SD in each group. Dotted black lines indicate the limit of quantification (LOQ). * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay